Optimised chromatographic conditions, system suitability, and recovery study. The chromatographic separations were achieved by RP-C18 column (250 mm × 4.6 mm, 5 μ), Shimadzu LC-20AT Prominence liquid chromatograph, and a mobile phase composed of water : methanol : glacial acetic acid (65 : 34 : 1 v/v). An isocratic RP-HPLC method was developed for the estimation of P-coumaric acid from methanolic extracts of durva grass. The P-coumaric acid was procured from Sigma Aldrich (Scheme 1). It shows no interference from other compounds. In addition, digital pH meter (Systronics model 802) is used for all pH measurements in this study. 20 μL of sample solution prepared from methanolic extract was injected and the area of peak was recorded duly maintaining the ambient experimental conditions as followed by the standard drug solutions. ... p-Coumaric acid, trans-4-Hydroxycinnamic acid Linear Formula: HOC 6 H 4 CH=CHCO 2 H. Molecular Weight: 164.16. The specimen was shade-dried and protected from sun light for several days not less than one month. 2020-10-31. Yen, “Modulation of hepatic phase II phenol sulfotransferase and antioxidant status by phenolic acids in rats,”, S. E. Soares, “Phenolic acids as antioxidants,”, R. J. Robbins, “Phenolic acids in foods: an overview of analytical methodology,”, C.-S. Yao, M. Lin, X. Liu, and Y.-H. Wang, “Stilbene derivatives from Gnetum cleistostachyum,”, M. C. Gálvez, C. G. Barroso, and J. Double beam UV-visible spectrophotometer (Systronics model 2203) with matched cuvettes was used in this study. P-coumaric acid is a nonflavonoid phenolic acid and is a major constituent of the species Cynodon dactylon Linn.
Accuracy studies were performed for P-coumaric acid at three different levels (25%, 50%, and 100%) and the mixtures were analyzed in triplicate by the proposed method.
Copyright © 2015 Ramadoss Karthikeyan et al. × 250 mm, 5-micron particle size). )", International Journal of Analytical Chemistry, vol. p-Coumaric acid is the most abundant isomer of the three in nature. The flow rate was 1.0 mL/min and the analyses of column effluents were performed using UV-visible detector at 310 nm.
The flow rate was 1.0 mL/min phase at the required flow rate to get a steady base line. There were few miscellaneous methods reported for determination of phenolic acid content [25–27]. Methanol, anhydrous sodium sulphate, calcium chloride, and so forth, were purchased from Merck Pvt. Molecular weights (MW) and important ions present in the mass spectra of P-coumaric acid in the examined plant extracts by ESI-MS. The amount of P-coumaric acid present in the sample of extract was computed from its calibration graph.
Ramadoss Karthikeyan, Chapala Devadasu, Puttagunta Srinivasa Babu, "Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) P-coumaric acid has antioxidant properties and is believed to reduce the risk of stomach cancer , by reducing the formation of carcinogenic nitrosamines .
It is also found in barley grain . A continuous Soxhlet apparatus (18 cm × 4.8 cm, Rankem, Mumbai) with heating mantle (1000 mL capacity) and vacuum filtration assembly (Baba, Mumbai) is used for extraction purpose.
Chromatograms showed a peak of P-coumaric acid at retention time of 6.617 min. TLC profile of compound was represented in Figure 1.
The UV spectrum of the purified compound was recorded from 190 to 600 nm on an ELICO double beam UV-visible spectrophotometer. An aliquot of standard solution of P-coumaric acid and a sample solution of crude extract are spotted onto the silica gel plate and allowed to dry for a few minutes. The (retention factor) values of isolated compounds and standard were calculated and compared. The whole plant was used for the research work. The effect of these variables on the developed method was determined. Glacial acetic acid, Methanol and water (HPLC grade) are also purchased from (Sigma Aldrich, Mumbai).
SDS; PHL89498 ; phyproof ® Reference Substance; pricing. The RF values of standard and sample compound match each other and the RF value was found as 0.52. The entire purification process was carried out under dark or dim light conditions. Characterisation of isolated compound was done by studying ultraviolet, mass, and H1 NMR spectra. The material is cleaned and set free from moulds, insects, animal faecal matter and other contaminations such as earth, stones, and extraneous materials. It is found in wine and vinegar . This compound is structurally related to the investigated compounds and behaves similarly on the column as analyte. It is found in wine and vinegar . PDC catalyzes the formation of the corresponding 4-vinyl derivatives (vinyl phenol, vinyl catechol, and vinyl guaiacol) from p-coumaric, caffeic, and ferulic acids, respectively (Rodríguez et … Du, “Pharmacokinetic study of P-coumaric acid in mouse after oral administration of extract of, Z. Spáčil, L. Nováková, and P. Solich, “Analysis of phenolic compounds by high performance liquid chromatography and ultra performance liquid chromatography,”, T. Zhang, D. Yang, Y. Wang, X. Chen, and K. Bi, “Simultaneous determination of four components in the herbs of, N. P. Geetha, M. Mahesh, B. K. Bettadaiah, R. K. Kini, and H. S. Prakas, “HPLC method for determination of P-coumaric acid from the medicinal herb Leptadinia reticulate,”, N. Ongphimai, S. Lilitchan, K. Aryusuk, A. Bumrungpert, and K. Krisnangkura, “Phenolic acids content and antioxidant capacity of fruit extracts from Thailand,”, S. I. Mussatto, G. Dragone, and I. C. Roberto, “Ferulic and, C. D. Stalikas, “Extraction, separation, and detection methods for phenolic acids and flavonoids,”, A. H. Laghari, S. Memon, A. Nelofar, K. M. Khan, and A. Yasmin, “Determination of free phenolic acids and antioxidant activity of methanolic extracts obtained from fruits and leaves of, R. Świsłocka, M. Kowczyk-Sadowy, M. Kalinowska, and W. Lewandowski, “Spectroscopic (FT-IR, FT-Raman, 1H and 13C NMR) and theoretical studies of, G. Rajauria and N. Abu-Ghannam, “Isolation and partial characterization of bioactive fucoxanthin from. Accurately about 20 μL of each calibration standard was injected into the chromatogram. Further the study also deals with characterisation of P-coumaric acid in the extract by mass and H1 NMR spectral analysis for structure identification. There are three isomers of coumaric acid—o-coumaric acid, m-coumaric acid, and p-coumaric acid—that differ by the position of the hydroxy substitution of the phenyl group. We are committed to sharing findings related to COVID-19 as quickly as possible. This study was aimed at development of suitable extraction and isolation procedure which aids in getting the purified material and in developing an isocratic RP-HPLC method for estimation of P-coumaric acid in methanolic extract of durva grass. In food P-coumaric acid can be found in a wide variety of edible plants such as peanuts, navy beans, tomatoes, carrots, and garlic. The whole plant Cynodon dactylon was used in this study. Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (, Vignan Pharmacy College, Vadlamudi, Guntur, Andhra Pradesh 522 213, India, International Journal of Analytical Chemistry, SHIMADZU LC-20AT Prominence liquid chromatograph, SHIMADZU SPD-20A Prominence UV-Vis detector, Water : methanol : glacial acetic acid (65 : 34 : 1 v/v), Amount of reference standard added to preanalysed samples (, N. C. Cook and S. Samman, “Flavonoids: chemistry, metabolism, cardioprotective effects and dietary sources,”, Y. Hanasaki, S. Ogawa, and S. Fukui, “The correlation between active oxygens scavenging and antioxidative effects of flavonoids,”, C.-P. Chung, J.-B.
Further the extracts were used for different studies. InChI=1S/C9H8O3/c10-8-4-1-7(2-5-8)3-6-9(11)12/h1-6,10H,(H,11,12)/b6-3+.
A. Pérez-Bustamante, “Analysis of polyphenolic compounds of different vinegar samples,”, Z. Quinde-Axtell and B.-K. Baik, “Phenolic compounds of barley grain and their implication in food product discoloration,”, L. R. Ferguson, S.-T. Zhu, and P. J. Harris, “Antioxidant and antigenotoxic effects of plant cell wall hydroxycinnamic acids in cultured HT-29 cells,”, K. Kikugawa, T. Hakamada, M. Hasunuma, and T. Kurechi, “Reaction of, W. R. Russell, A. Labat, L. Scobbie, G. J. Duncan, and G. G. Duthie, “Phenolic acid content of fruits commonly consumed and locally produced in Scotland,”, X.-Q.
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